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Cell Culture and Biotechnology for Tissue Engineering
OVERVIEW
CEA CAPA Partner Institution: Universidad Carlos III de Madrid
Location: Madrid, Spain
Primary Subject Area: Biomedical Engineering
Instruction in: English
Transcript Source: Partner Institution
Course Details: Level 400
Recommended Semester Credits: 3
Contact Hours: 42
Prerequisites: The student must have coursed Introduction to Bioengineering, Cell and Molecular biology, Biochemistry, Biomechanics of continuum media I (solids), Biomechanics of continuum media II (fluids) Fundamentals of Tissue engineering and Regenerative
medicine, Transport phenomena in Biomedicine, and Introduction to Biomaterials.
DESCRIPTION
PROGRAMME:
1. Original tissue properties, study of cultured tissues properties.
2. Prokaryotic and eukaryotic cell culture.
3. Non-human cell cultures: Biotechnological applications.
4. Human cell culture: allogeneic, autologous and xenogenic cells: transplantation and rejection
5. Human stem cell cultures: Embryonic and adult stem cells. iPS.
6. Tissue culture in 2D and 3D.
7. Bioreactors.
8. Tissue mechanics and Mechanotransduction.
9. Tissue on a chip, Organ on a chip and human on a chip (disease modeling).
10. Recombinant DNA technology: Recombinant growth factor (morphogens and angiogenic factors).
11. Genetic engineering: transgenic animals as biofactories and gene therapy.
12. Current applications and Goverment regulations for Engineered tissues.
LABORATORY EXPERIMENTS: Cell Culture Laboratory for Biotechnology and Tissue Engineering.
a. Experimental design and methods that will be useful for tissue characterization and development
b. Understand biomaterial generation for organ bioengineering.
c. Stem cell harvest and isolation.
d. Tissue/organ bioengineering
d. Tissues histological and molecular characterization using biotechnological tools.
e. Critical evaluation of scientific results
1. Original tissue properties, study of cultured tissues properties.
2. Prokaryotic and eukaryotic cell culture.
3. Non-human cell cultures: Biotechnological applications.
4. Human cell culture: allogeneic, autologous and xenogenic cells: transplantation and rejection
5. Human stem cell cultures: Embryonic and adult stem cells. iPS.
6. Tissue culture in 2D and 3D.
7. Bioreactors.
8. Tissue mechanics and Mechanotransduction.
9. Tissue on a chip, Organ on a chip and human on a chip (disease modeling).
10. Recombinant DNA technology: Recombinant growth factor (morphogens and angiogenic factors).
11. Genetic engineering: transgenic animals as biofactories and gene therapy.
12. Current applications and Goverment regulations for Engineered tissues.
LABORATORY EXPERIMENTS: Cell Culture Laboratory for Biotechnology and Tissue Engineering.
a. Experimental design and methods that will be useful for tissue characterization and development
b. Understand biomaterial generation for organ bioengineering.
c. Stem cell harvest and isolation.
d. Tissue/organ bioengineering
d. Tissues histological and molecular characterization using biotechnological tools.
e. Critical evaluation of scientific results
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