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Single-Cell Technologies in Life Sciences
OVERVIEW
CEA CAPA Partner Institution: Vrije Universiteit Amsterdam
Location: Amsterdam, Netherlands
Primary Subject Area: Health Sciences
Other Subject Area: Biology
Instruction in: English
Transcript Source: Partner Institution
Course Details: Level 400
Recommended Semester Credits: 3
Contact Hours: 45
DESCRIPTION
Life Sciences research at the level of individual cells is a rapidly developing research area. Traditional single-cell technologies such as cytometry and microscopy are being used in combination with the emerging field of single-cell genomics. These single-cell technologies have important implications for our understanding of cellular behaviour, including cellular development, differentiation and decision-making. Cellular processes can now be studied at an unprecedented resolution, and with astounding results: new light can now be shed on immunity and immune-related disorders, infectious diseases, and cancer. Understanding the technology behind these new applications is crucial to the processing and interpretation of data.
This intensive course combines theory with practice and will provide participants with in-depth and up-to-date expertise on advanced cytometry and microscopic imaging techniques. It is sure to be invaluable to everyone who is planning to complete a research internship. The course is divided into two parts: cytometry and microscopy. The cytometry lectures will teach students the principles of fluorescence cytometry analysis and sorting, imaging flow cytometry, and mass cytometry. Students will also be required to attend a demonstration session on cytometry and will learn all about essential data analysis methods (from multicolour cytometry to multidimensional data analysis) through tutorials and work group sessions. This part of the course will include an exciting lecture on the emerging field of single-cell genomics. The second part of the course will focus on light microscopy on live and fixed specimens, confocal laser scanning microscopy, electron microscopy, and light-sheet microscopy. Special attention will be given to image analysis in a tutorial on ImageJ and other commonly used software applications.
Contact hours listed under a course description may vary due to the combination of lecture-based and independent work required for each course. CEA's recommended credits are based on the contact hours assigned by Vrije Universiteit Amsterdam (VU Amsterdam): 15 contact hours equals 1 U.S. credit
This intensive course combines theory with practice and will provide participants with in-depth and up-to-date expertise on advanced cytometry and microscopic imaging techniques. It is sure to be invaluable to everyone who is planning to complete a research internship. The course is divided into two parts: cytometry and microscopy. The cytometry lectures will teach students the principles of fluorescence cytometry analysis and sorting, imaging flow cytometry, and mass cytometry. Students will also be required to attend a demonstration session on cytometry and will learn all about essential data analysis methods (from multicolour cytometry to multidimensional data analysis) through tutorials and work group sessions. This part of the course will include an exciting lecture on the emerging field of single-cell genomics. The second part of the course will focus on light microscopy on live and fixed specimens, confocal laser scanning microscopy, electron microscopy, and light-sheet microscopy. Special attention will be given to image analysis in a tutorial on ImageJ and other commonly used software applications.
Contact hours listed under a course description may vary due to the combination of lecture-based and independent work required for each course. CEA's recommended credits are based on the contact hours assigned by Vrije Universiteit Amsterdam (VU Amsterdam): 15 contact hours equals 1 U.S. credit
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